service - Cryo-EM SPA
B A C KCryo-EM Services for High Resolution Protein Structure Determination
In the past few years, single-particle cryo-EM in particular has triggered a revolution in structural biology and has become a newly dominant discipline.Today，Single Particle Analysis（SPA）is no longer a complementary technique but a dominant one, changing the field of structural biology in a profound and unprecedented way and facilitating major new discoveries.
Cryo-EM SPA Workflow
Why do you need Cryo-EM SPA Services?
● small amount
● a large biomolecular complex
● hard to crystallize
More far-reaching significance：
● Pursuing highly innovative protein structure analysis
● Revealing complex conformational mechanisms in the process of protein function
● Advance the design and optimization of next-generation inhibitors, regulators and other related ligands
Why choose ShuimuBio for Cryo-EM SPA?
ShuimuBio hosts two high-end cryo-electron microscopes and established powerful, stable, and versatile system for delivering high-resolution 3D structure of biological samples in their near-native state. Our cryo-EM services are designed to support a full range of project types from data acquisition to project-based proposal. We are able to complete a structure determination project within two to three weeks for well-behaved samples.
About the samples
Protein （50-100 ul，≥2mg/mL，Purity >95%）:
SDS-PAGE without obvious stray bands and degradation bands, also to ensure that it is the target protein (mass spectrometry identification, etc.).
Buffer （50-100 ml）:
Do not contain organic substances such as polysaccharides, DMSO, glycerol (otherwise it will affect the lining), salt ion concentration below 300 mM.
Homogeneity:
Before negative staining, try to do gel filtration chromatography on the sample (this step is necessary if it is a complex), if the result shows a single peak, it proves that the sample homogeneity is greater than 90%. (Glycosylation or phosphorylation modifications have less effect on the electron microscopic structure resolution); after gel filtration layer, do not concentrate again to avoid protein aggregation, etc.
Storage (10ul-20ul/tube):
It is recommended that samples be dispensed directly after preparation to avoid the need for repeated freezing and thawing during the experiment, resulting in unusable samples.